Insights into qPCR: Protocol, Detection Methods, and Analysis
Nov 8, 2023 · RT-PCR vs. qPCR. Reverse transcription PCR (RT-PCR) is often confused with real-time PCR. RT-PCR utilizes a reverse transcriptase enzyme to convert ribonucleic acid (RNA) into complementary DNA (cDNA). 1 When combined with qPCR, the method is called reverse transcription real-time quantitative PCR . 3 RT-qPCR is a powerful tool because it enables researchers to measure gene expression of ...
What Does a Positive Covid Test Look Like? - The Scientist …
May 10, 2024 · ABOVE: A positive rapid test will yield two visible lines (middle) while a valid negative test will only show a single line in the control region (right) and an invalid test will show no control line (left). iStock, ASIFE V iral TikTok videos showing teenagers hacking rapid COVID-19 tests by inserting sodas and other acidic solutions into the tests’ inlet caused quite a …
Words for Nerds - Science Wordle Game - The Scientist Magazine®
It’s Like Science Wordle. Welcome to Words for Nerds, our popular and addictive daily science word game. Think of it as science Wordle—a guessing jumble for every nerd, especially the busy life scientist looking to get a little break from the rigors of research.
Artificial Organs: Innovating to Replace Donors and Dialysis
Jan 20, 2023 · Stay up to date on the latest science with Brush Up Summaries. What Are Artificial Organs? Artificial organs are bioengineered devices or tissues that scientists create and integrate into the human body to replace, duplicate, or augment functional, naturally occurring organs. 1 They pose a solution to organ donor shortages, and can also be used as medical training tools. 2
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Feb 23, 2024 · Template DNA. Template amount, source, and secondary structures or GC-rich regions may affect the reaction. 7. The amount of template depends on the DNA copy number. For a standard PCR reaction with 25-30 cycles, roughly 10 4 copies of the template DNA are sufficient to generate a detectable amount of PCR product. 7; The DNA required for a given reaction also depends on the source.
How Cloud Labs and Remote Research Shape Science
Mar 18, 2024 · Inception and Development of Remote Labs for Scientific Research. The idea of remote work can be traced back to the 1970s when Jack Nilles, a NASA engineer, proposed the use of telecommuting to alleviate traffic congestion. 4 The advent of personal computers and widespread internet access in the early 2000s has contributed to the concept of remote work being popularly accepted as a viable ...
Interstitium: A Network of Living Spaces Supports Anatomical ...
Apr 26, 2024 · The interstitium is continuous throughout tissue, in this case, the human pancreas, as well as the entire body. The image on the left shows a cross section through the draining duct of a human pancreas surrounded by thick bundles of supportive collagen networks in red.
Innovation Spotlight | The Scientist Magazine®
May 15, 2023 · The Scientist's Innovation Spotlight articles. Kevin Fitzgerald from Alnylam Pharmaceuticals explains the potential of RNA interference for treating disease and highlights recent clinical trial successes.
Sketching out Cell Theory, circa 1837 | The Scientist Magazine®
Aug 1, 2013 · Robert Hooke had coined the word “cell”—short for cellula, or “small compartment” in Latin—in the mid-17th century, after he’d seen tiny rectangular shapes while studying slices of cork using a rudimentary microscope.
Tracking Community Health Through Wastewater Surveillance
Feb 1, 2024 · Detecting infectious diseases. In 1939, scientists detected live poliovirus within wastewater during a polio (also known as poliomyelitis) epidemic. 3 However, they were unable to detect these viral particles after the epidemic ended. 4 They hypothesized that researchers could use wastewater testing to track infection levels within a …