For filter-aided sample preparation (FASP), a Microcon-10 kDa centrifugal filter unit with Ultracel-10 membranes (ultrafilter) were used, and for solid phase extraction (SPE), Pierce C18 Spin Tips and ...

We developed a high efficiency enrichment method for oxidized phospholipid-modified peptides that employs a C18 SPE column. Using this approach, in combination with LC-MS analysis, we studied the ...

The online sample loading volume of 5 mL was achieved at a 2,500 μL/min flow rate into a Thermo Hypersil Gold aQ column (C18, 20 mm × 2 mm, 12 μm particle size) hereafter named online SPE column.

In this research, the composition and content of polyphenols in ten samples of linden honey from northeast China and eastern Russia were determined by using solid-phase extraction method combined with ...

The digested peptides were desalted by the C18-Reverse-Phase SPE Column. RP separation was performed on an 1100 HPLC System (Agilent) using an Agilent Zorbax Extend RP column (5 μm, 150 mm × 2.1 mm).

Today, solid-phase extraction (SPE) has become a widely acclaimed sample preparation tool for analytical chemists. This technique was developed as an alternative to liquid-liquid extraction (LLE).

Conclusion All the experiments described above indicate that SPE using a C18 resin is a viable approach for removing phospholipids.